Using short-term bioassays to evaluate the endocrine disrupting capacity of the pesticides linuron and fenoxycarb.
Identifieur interne : 000089 ( Main/Exploration ); précédent : 000088; suivant : 000090Using short-term bioassays to evaluate the endocrine disrupting capacity of the pesticides linuron and fenoxycarb.
Auteurs : Petra Spirhanzlova [France] ; Bert De Groef [Australie] ; Freda E. Nicholson [Australie] ; Sylvia V H. Grommen [Australie] ; Giulia Marras [France] ; Anthony Sébillot [France] ; Barbara A. Demeneix [France] ; Sophie Pallud-Mothré [France] ; Gregory F. Lemkine [France] ; Andrew J. Tindall [France] ; David Du Pasquier [France]Source :
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP [ 1532-0456 ] ; 2017.
Abstract
Several short-term whole-organism bioassays based on transgenic aquatic models are now under validation by the OECD (Organization for Economic Co-operation and Development) to become standardized test guidelines for the evaluation of the endocrine activity of substances. Evaluation of the endocrine disrupting capacity of pesticides will be a domain of applicability of these future reference tests. The herbicide linuron and the insecticide fenoxycarb are two chemicals commonly used in agricultural practices. While numerous studies indicate that linuron is likely to be an endocrine disruptor, there is little information available on the effect of fenoxycarb on vertebrate endocrine systems. Using whole-organism bioassays based on transgenic Xenopus laevis tadpoles and medaka fry we assessed the potential of fenoxycarb and linuron to disrupt thyroid, androgen and estrogen signaling. In addition we used in silico approach to simulate the affinity of these two pesticides to human hormone receptors. Linuron elicited thyroid hormone-like activity in tadpoles at all concentrations tested and, showed an anti-estrogenic activity in medaka at concentrations 2.5mg/L and higher. Our experiments suggest that, in addition to its previously established anti-androgenic action, linuron exhibits thyroid hormone-like responses, as well as acting at the estrogen receptor level to inhibit estrogen signaling. Fenoxycarb on the other hand, did not cause any changes in thyroid, androgen or estrogen signaling at the concentrations tested.
DOI: 10.1016/j.cbpc.2017.06.006
PubMed: 28634053
Affiliations:
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Grommen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Physiology, Anatomy and Microbiology, La Trobe University, Bundoora, Victoria 3086, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Physiology, Anatomy and Microbiology, La Trobe University, Bundoora, Victoria 3086</wicri:regionArea><wicri:noRegion>Victoria 3086</wicri:noRegion></affiliation></author><author><name sortKey="Marras, Giulia" sort="Marras, Giulia" uniqKey="Marras G" first="Giulia" last="Marras">Giulia Marras</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire WatchFrog S.A., 1 Rue Pierre Fontaine, 91000 Évry, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire WatchFrog S.A., 1 Rue Pierre Fontaine, 91000 Évry</wicri:regionArea><wicri:noRegion>91000 Évry</wicri:noRegion><wicri:noRegion>91000 Évry</wicri:noRegion></affiliation></author><author><name sortKey="Sebillot, Anthony" sort="Sebillot, Anthony" uniqKey="Sebillot A" first="Anthony" last="Sébillot">Anthony Sébillot</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire WatchFrog S.A., 1 Rue Pierre Fontaine, 91000 Évry, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire WatchFrog S.A., 1 Rue Pierre Fontaine, 91000 Évry</wicri:regionArea><wicri:noRegion>91000 Évry</wicri:noRegion><wicri:noRegion>91000 Évry</wicri:noRegion></affiliation></author><author><name sortKey="Demeneix, Barbara A" sort="Demeneix, Barbara A" uniqKey="Demeneix B" first="Barbara A" last="Demeneix">Barbara A. Demeneix</name><affiliation wicri:level="3"><nlm:affiliation>UMR CNRS 5166, Evolution des Régulations Endocriniennes, Department of Regulation, Development and Molecular Diversity, Muséum National d'Histoire Naturelle, Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UMR CNRS 5166, Evolution des Régulations Endocriniennes, Department of Regulation, Development and Molecular Diversity, Muséum National d'Histoire Naturelle, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Pallud Mothre, Sophie" sort="Pallud Mothre, Sophie" uniqKey="Pallud Mothre S" first="Sophie" last="Pallud-Mothré">Sophie Pallud-Mothré</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire WatchFrog S.A., 1 Rue Pierre Fontaine, 91000 Évry, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire WatchFrog S.A., 1 Rue Pierre Fontaine, 91000 Évry</wicri:regionArea><wicri:noRegion>91000 Évry</wicri:noRegion><wicri:noRegion>91000 Évry</wicri:noRegion></affiliation></author><author><name sortKey="Lemkine, Gregory F" sort="Lemkine, Gregory F" uniqKey="Lemkine G" first="Gregory F" last="Lemkine">Gregory F. 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Tindall</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire WatchFrog S.A., 1 Rue Pierre Fontaine, 91000 Évry, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire WatchFrog S.A., 1 Rue Pierre Fontaine, 91000 Évry</wicri:regionArea><wicri:noRegion>91000 Évry</wicri:noRegion><wicri:noRegion>91000 Évry</wicri:noRegion></affiliation></author><author><name sortKey="Du Pasquier, David" sort="Du Pasquier, David" uniqKey="Du Pasquier D" first="David" last="Du Pasquier">David Du Pasquier</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire WatchFrog S.A., 1 Rue Pierre Fontaine, 91000 Évry, France. Electronic address: pasquier@watchfrog.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire WatchFrog S.A., 1 Rue Pierre Fontaine, 91000 Évry</wicri:regionArea><wicri:noRegion>91000 Évry</wicri:noRegion><wicri:noRegion>91000 Évry</wicri:noRegion></affiliation></author></analytic><series><title level="j">Comparative biochemistry and physiology. Toxicology & pharmacology : CBP</title><idno type="ISSN">1532-0456</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Several short-term whole-organism bioassays based on transgenic aquatic models are now under validation by the OECD (Organization for Economic Co-operation and Development) to become standardized test guidelines for the evaluation of the endocrine activity of substances. Evaluation of the endocrine disrupting capacity of pesticides will be a domain of applicability of these future reference tests. The herbicide linuron and the insecticide fenoxycarb are two chemicals commonly used in agricultural practices. While numerous studies indicate that linuron is likely to be an endocrine disruptor, there is little information available on the effect of fenoxycarb on vertebrate endocrine systems. Using whole-organism bioassays based on transgenic Xenopus laevis tadpoles and medaka fry we assessed the potential of fenoxycarb and linuron to disrupt thyroid, androgen and estrogen signaling. In addition we used in silico approach to simulate the affinity of these two pesticides to human hormone receptors. Linuron elicited thyroid hormone-like activity in tadpoles at all concentrations tested and, showed an anti-estrogenic activity in medaka at concentrations 2.5mg/L and higher. Our experiments suggest that, in addition to its previously established anti-androgenic action, linuron exhibits thyroid hormone-like responses, as well as acting at the estrogen receptor level to inhibit estrogen signaling. Fenoxycarb on the other hand, did not cause any changes in thyroid, androgen or estrogen signaling at the concentrations tested.</div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li></country><region><li>Île-de-France</li></region><settlement><li>Paris</li></settlement></list><tree><country name="France"><region name="Île-de-France"><name sortKey="Spirhanzlova, Petra" sort="Spirhanzlova, Petra" uniqKey="Spirhanzlova P" first="Petra" last="Spirhanzlova">Petra Spirhanzlova</name></region><name sortKey="Demeneix, Barbara A" sort="Demeneix, Barbara A" uniqKey="Demeneix B" first="Barbara A" last="Demeneix">Barbara A. Demeneix</name><name sortKey="Du Pasquier, David" sort="Du Pasquier, David" uniqKey="Du Pasquier D" first="David" last="Du Pasquier">David Du Pasquier</name><name sortKey="Lemkine, Gregory F" sort="Lemkine, Gregory F" uniqKey="Lemkine G" first="Gregory F" last="Lemkine">Gregory F. Lemkine</name><name sortKey="Marras, Giulia" sort="Marras, Giulia" uniqKey="Marras G" first="Giulia" last="Marras">Giulia Marras</name><name sortKey="Pallud Mothre, Sophie" sort="Pallud Mothre, Sophie" uniqKey="Pallud Mothre S" first="Sophie" last="Pallud-Mothré">Sophie Pallud-Mothré</name><name sortKey="Sebillot, Anthony" sort="Sebillot, Anthony" uniqKey="Sebillot A" first="Anthony" last="Sébillot">Anthony Sébillot</name><name sortKey="Tindall, Andrew J" sort="Tindall, Andrew J" uniqKey="Tindall A" first="Andrew J" last="Tindall">Andrew J. 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